DBscript 2.0 Reverse Transcriptase: Ultra-sensitive RT

DBscript 2.0 Reverse Transcriptase: Ultra-sensitive M-MLV mutant

(DB-1332)

Upgrade your reverse transcription workflow with DBscript 2.0 Reverse Transcriptase, an advanced, ultra-sensitive M-MLV mutant. Engineered to outperform standard enzymes, DBscript 2.0 delivers ~10-fold faster reaction times and higher thermostability than every competitor on the market.

Whether you are performing routine syntheses or detecting low copy number targets down to ~1 copy, this robust enzyme ensures reliable, maximum-yield cDNA synthesis for all your critical 1-step RT-PCR and diagnostic applications.

Package size

Key Parameters: The best solution for most applications

Maximum Sensitivity: Specifically optimized for low copy number targets, successfully detecting down to ~1 copy.

Exceptional Speed: Performs ~10-fold faster than Superscript III or III Flash.

High Thermostability: Features an optimum activity range of 50-60°C and maintains functionality for RT steps at up to 65°C.

Uncompromised Yield: Consistently delivers the same or better cDNA yield when compared to Superscripts.

General info

Name (Cat. no.)	Quantity	Lead Time
DBscript 2.0 Reverse Transcriptase (DB-1332)	2 kU	In Stock
DBscript 2.0 Reverse Transcriptase (DB-1332)	10 kU	In Stock
DBscript 2.0 Reverse Transcriptase (DB-1332)	40 kU	In Stock

Details

Source	Unit Definition	Storage Buffer	QC Assays
Recombinant E. coli	One unit is defined as the amount of enzyme that catalyzes incorporation of 1 nmol dNTPs (detected with SYBR® Green I dye) into 100 nM extendable DNA-RNA heteroduplex hairpin within 10 min at 37 °C.	20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40, 50 % glycerol	SDS-PAGE/purity Functional Assay (cDNA synthesis)

Source

Unit Definition

Storage Buffer

QC Assays

Recombinant E. coli

One unit is defined as the amount of enzyme that catalyzes incorporation of 1 nmol dNTPs (detected with SYBR® Green I dye) into

100 nM extendable DNA-RNA heteroduplex hairpin within 10 min at 37 °C.

20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40,

50 % glycerol

SDS-PAGE/purity

Functional Assay (cDNA synthesis)

Downloads

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DBscript shows higher cDNA yield than its competitors

cDNA yield after 5 min preincubation at various temperatures:Comparison of approximate cDNA yield reflecting enzyme stability after a 5-minute preincubation at various temperatures (Reaction conditions: 10-minute synthesis step at 55°C; 0.3 U/µL enzyme concentration; 500-base transcript). DBscript 2.0: Demonstrates outstanding resilience, retaining full cDNA yield even after a stringent 60°C preincubation, enabling higher reaction temperatures to ensure vastly improved sensitivity. DBscript Turbo: Delivers extreme thermal endurance, easily surviving 60°C preincubation with maximum yield and allowing for RT steps at 50–60°C even for longer, complex transcripts.

DBscript 2.0 and DBscript Turbo: Significantly faster protocols

Accelerated cDNA Synthesis Speed Comparison:Comparison of approximate cDNA yield between DIANA DBscript and Thermo SuperScript™ enzymes across 1-minute and 10-minute reaction times. Ultra-Fast Synthesis (1 min): In a rapid 1-minute reaction, DBscript 2.0 and DBscript Turbo operate >10x and >100x faster, respectively, than baseline SuperScript™ III, generating substantial yields before standard enzymes register significant activity. Maximum Yield (10 min): After a standard 10-minute incubation, the engineered DIANA mutants demonstrate profound dominance at elevated temperatures. At 60°C, both DBscript 2.0 and DBscript Turbo reach near-maximum yields (up to ~100%), drastically outperforming SuperScript™ IV and SuperScript™ III Flash.

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