(DB-1353)
Prevent RT-PCR carry-over contamination without compromising assay performance. DB Thermolabile UDG for RT-PCR 100X is a specialized thermolabile enzyme optimized for RT-qPCR workflows. It selectively removes uracil-containing DNA originating from previous amplification reactions, helping eliminate false-positive results caused by cross-contamination.
Unlike conventional UDG enzymes, DB Thermolabile UDG remains fully compatible with reverse transcription at 50°C or higher while delivering outstanding stability in reaction mixes at room temperature and during freeze-thaw cycles. This unique combination makes it an ideal choice for qPCR workflows requiring robust contamination prevention.
DB Thermolabile UDG for PCR is now available in a Lyo-Ready formulation for seamless integration into lyophilized qPCR and diagnostic reagent mixes.
Package size
DB Thermolabile UDG for RT-PCR Lyo-Ready 100x: Prevents carry-over contamination during RT-qPCR
Twice as active as cod UDG at Room-Temperature: Unlike conventional thermolabile UDG enzymes, DB Thermolabile UDG remains stable in RT-qPCR reaction mixes at room temperature, simplifying assay preparation, transport, and routine laboratory workflows.
Effective Carry-Over Contamination Control: Selectively removes uracil-containing DNA from previous PCR reactions, helping eliminate false-positive results and improving confidence in amplification data.
Optimized for RT-qPCR Applications: Designed for reliable contamination control in quantitative RT-PCR workflows without compromising amplification performance.
Lyo-Ready Format: Offered as a glycerol-free Lyo-Ready version to support lyophilized assay development and manufacturing.
General info
Name (Cat. no.) | Quantity | Lead Time |
|---|---|---|
DB Thermolabile UDG for RT-PCR Lyo-Ready 100X (DB-1353) | 200 rxns | 1–2 days (CZ) / within 7 days (EU) |
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Czechia, Slovakia and Hungary | UK and Ireland | Italy | Germany, Switzerland and Austria | Denmark, Sweden, Finland, Island and Norway | Benelux, Spain and Portugal |
|---|---|---|---|---|---|
Details
Source | Unit Definition | Storage Buffer | QC Assays |
|---|---|---|---|
Recombinant E. coli | One unit is defined as the amount of enzyme that catalyzes the release of 1 nmol of uracil within 60 min at 37 °C from 200 nM uracil-containing fluorophore-quencher dual-labelled DNA molecular beacon. | 20 mM Tris 7.5, 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 1 mg/ml BSA | Activity: the enzyme activity is measured for each lot and determined according to the unit definition. Purity: SDS-PAGE shows at least 90 % purity. |
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