DBdirect RT-PCR SYBR Mix (DB-1265)

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DBdirect™ RT-PCR SYBR Mix (DB-1265)

DBdirect™ RT-PCR SYBR mix contains SYBR® Green I dye for real-time quantification of RNA sequences and offers an ideal solution for routine and high-throughput RNA target detection. Meet the advantages of direct RT-PCR with no need for RNA extraction!

This system has been used as a basis in millions of diagnostic tests against COVID-19 including direct detection from samples of human saliva or standard collection media without the need for a previous RNA isolation step. Example of direct detection of viral RNA in cell culture is described in bit.ly/pubmed-DB-1.

DBdirect RT-PCR SYBR Mix (DB-1265)_photo

Ready to use mix for fast and comfortable workflows with the DBdirect™ Technology!

SYBR® Green I dye included in the mix: No TaqMan probes are needed

Hot-start: Aptamer

Various applications including qualitative and quantitative real-time RT-PCR from RNA

Detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media)

Name (Cat. no.)
Lead Time
DBdirect™ RT-PCR SYBR Mix (DB-1265)
100 RXNS
90 EUR
In Stock
DBdirect™ RT-PCR SYBR Mix (DB-1265)
1000 RXNS
590 EUR
In Stock
DBdirect™ RT-PCR SYBR Mix (DB-1265)
5000 RXNS
2360 EUR
In Stock

Key Proteins, Hot-start, Content and QC

Key Proteins

Taq DNA Polymerase
Thermostable reverse transcriptase (mutated M-MLV)
RNase Inhibitors




Enhancer mix (4x)
RT-PCR SYBR Mix (4x)
PCR Grade Water

QC Assays

Functional test (RT-PCR)
E. coli gDNA absence
RNase and DNase absence
Activity measurement of key proteins


Order DBdirect RT-PCR SYBR Mix or ask for a free sample:


    Aleš Pauk

    Head of Sales 

    Aleš Pauk Head of Sales