DBdirect™ RT-PCR SYBR Mix (DB-1265)
DBdirect™ RT-PCR SYBR mix contains SYBR® Green I dye for real-time quantification of RNA sequences and offers an ideal solution for routine and high-throughput RNA target detection. Meet the advantages of direct RT-PCR with no need for RNA extraction!
This system has been used as a basis in millions of diagnostic tests against COVID-19 including direct detection from samples of human saliva or standard collection media without the need for a previous RNA isolation step. Example of direct detection of viral RNA in cell culture is described in bit.ly/pubmed-DB-1.
Ready to use mix for fast and comfortable workflows with the DBdirect™ Technology!
SYBR® Green I dye included in the mix: No TaqMan probes are needed
Hot-start: Aptamer
Various applications including qualitative and quantitative real-time RT-PCR from RNA
Detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media)
Name (Cat. no.) | Quantity | Price | Lead Time |
DBdirect™ RT-PCR SYBR Mix (DB-1265) | 100 RXNS | 90 EUR | In Stock |
DBdirect™ RT-PCR SYBR Mix (DB-1265) | 1000 RXNS | 590 EUR | In Stock |
DBdirect™ RT-PCR SYBR Mix (DB-1265) | 5000 RXNS | 2360 EUR | In Stock |
Key Proteins, Hot-start, Content and QC
Key Proteins
Taq DNA Polymerase
Thermostable reverse transcriptase (mutated M-MLV)
RNase Inhibitors
Hot-start
Aptamer-based
Content
Enhancer mix (4x)
DBdirect™ RT-PCR SYBR Mix (4x)
PCR Grade Water
QC Assays
Functional test (RT-PCR)
E. coli gDNA absence
RNase and DNase absence
Activity measurement of key proteins
Downloads:
Order DBdirect™ RT-PCR SYBR Mix or ask for a free sample:
Aleš Pauk
Head of Sales
