DIANA Biotechnologies | DBScript cDNA Synthesis Kit

DBscript cDNA Synthesis Kit

(DB-1285)

Complete cDNA synthesis Kit with our advanced kit, featuring a unique bovine RNase inhibitor and DBscript Reverse Transcriptase—an MMLV mutant designed for higher yield and synthesis of longer cDNAs (> 9 kb).

Enjoy superior performance with reverse transcription at 50-60 °C, made possible by enhanced protein thermostability and our exclusive buffer formulation. Benefit from an ultra-fast 10-minute protocol for two-step RT-PCR and optimized buffers for seamless one-step RT-PCR.

Package size

Key Parameters: Complete cDNA synthesis with our unique bovine RNase inhibitor

DBscript Reverse Transcriptase with wide range sensitivity: Analyze 1 μg to 1 pg amount of RNA with 2-step RT-PCR

Reverse transcription at temperatures from 50 to 60 °C due to a combination of increased thermostability of the protein and a unique buffer formulation. Long-term sample storage.

Short protocol (10 min) for two-step RT-PCR. Optimal protocol and buffer for one-step RT-PCR. Recombinant expression in E. coli: no risk of transmission of infectious agents.

DB RNase Inhibitor Bovine with exceptional oxidation resistance (incl.).

Pricing

Name (Cat. no.)	Quantity	Price	Lead Time
DBscript cDNA Synthesis Kit (DB-1285)	50 rxns	180 EUR	In Stock

Details

Source	Content	Storage Buffer	QC Assays
Recombinant E. coli	DBscript Reverse Trancriptase (DB-1276) DB RNase Inhibitor Bovine (DB-1260) Oligo(dT)20 primer, random hexamers, RT Buffer A (5X), and a separate DTT solution (20X)	20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40, 50 % glycerol	SDS-PAGE/purity, DNase, RNase, latent RNase, endonuclease, DNA content absence, Functional Assay (cDNA synthesis)

Source

Content

Storage Buffer

QC Assays

Recombinant E. coli

DBscript Reverse

Trancriptase (DB-1276)

DB RNase Inhibitor Bovine (DB-1260)

Oligo(dT)20 primer, random hexamers, RT Buffer A (5X), and a separate DTT solution (20X)

20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40,

50 % glycerol

SDS-PAGE/purity, DNase, RNase, latent RNase, endonuclease, DNA content absence,

Functional Assay (cDNA synthesis)

DBscript protocol is faster than its direct competitors

Efficient Reverse Transcription with DBscript Reverse Transcription:DBscript cDNA Synthesis Kit, powered by our thermostable DBscript Reverse Transcriptase, offers the shortest protocol time among top competitors. Its streamlined workflow accelerates primer annealing, incubation, and cDNA synthesis, without compromising performance. For researchers demanding precision and efficiency, DBscript is the optimal choice, minimizing time while maximizing output. This kit exemplifies our commitment to advancing molecular biology with faster, reliable results.

DBscript: Sensitive and efficient

DBscript is sensitive and efficient with wide range of RNA input:To demonstrate the performance of DBscript Reverse Transcriptase in 2-step RT-qPCR setting, total RNA isolated from HEK cells was used with input amounts ranging from 1 µg to 1 pg. This corresponds to approximately 44,000 cells down to 0.04 cells or 4,400,000 RNA transcripts down to just 4.4 transcripts, assuming 100 copies of GAPDH RNA per cell. Three parallel sets of cDNA synthesis reactions were carried out with the following primers: Oligo(dT)20-VN (DB-1283; 2.5 µM), Random Hexamers (DB-1284; 1.25 µM), and a gene-specific primer for GAPDH detection (0.1 µM). Reactions were performed at 50 °C for 10 minutes, followed by termination at 94 °C for 2 minutes. Each reaction used 200 units of DBscript Reverse Transcriptase. Subsequently, 2 µl of cDNA were amplified in a PCR reaction using AptaTaq DNA Polymerase (DB-1277), primers, and a probe specific for GAPDH. The results demonstrate a linear sensitivity of cDNA synthesis over the entire range of RNA input amounts, from 1 µg to 1 pg. This underscores the versa

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Reverse Transcriptase

DBscript Reverse Transcriptase is essential for the synthesis of the complementary DNA (cDNA) strand from the RNA template. Use our Reverse Transcriptase in both two-step and one-step RT-PCR to ensure excellent results in your applications.

DB Oligo(dT)20 Primer

Make your cDNA synthesis kit with our proprietary DB Oligo(dT)20 Primer.

DB Anchored Oligo(dT)20-VN Primer

Make your cDNA synthesis kit with our proprietary DB Anchored Oligo(dT)20-VN Primer.

Let's take your PCR to the next level!

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