(DB-1285)
Complete cDNA synthesis Kit with our advanced kit, featuring a unique bovine RNase inhibitor and DBscript Reverse Transcriptase—an MMLV mutant designed for higher yield and synthesis of longer cDNAs (> 9 kb).
Enjoy superior performance with reverse transcription at 50-60 °C, made possible by enhanced protein thermostability and our exclusive buffer formulation. Benefit from an ultra-fast 10-minute protocol for two-step RT-PCR and optimized buffers for seamless one-step RT-PCR.
Package size
Key Parameters: Complete cDNA synthesis with our unique bovine RNase inhibitor
DBscript Reverse Transcriptase with wide range sensitivity: Analyze 1 μg to 1 pg amount of RNA with 2-step RT-PCR
Reverse transcription at temperatures from 50 to 60 °C due to a combination of increased thermostability of the protein and a unique buffer formulation. Long-term sample storage.
Short protocol (10 min) for two-step RT-PCR. Optimal protocol and buffer for one-step RT-PCR. Recombinant expression in E. coli: no risk of transmission of infectious agents.
DB RNase Inhibitor Bovine with exceptional oxidation resistance (incl.).
Pricing
Name (Cat. no.) | Quantity | Price | Lead Time |
---|---|---|---|
DBscript cDNA Synthesis Kit (DB-1285) | 50 rxns | 180 EUR | In Stock |
Details
Source | Content | Storage Buffer | QC Assays |
---|---|---|---|
Recombinant E. coli | DBscript Reverse Trancriptase (DB-1276) DB RNase Inhibitor Bovine (DB-1260) Oligo(dT)20 primer, random hexamers, RT Buffer A (5X), and a separate DTT solution (20X) | 20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40, 50 % glycerol | SDS-PAGE/purity, DNase, RNase, latent RNase, endonuclease, DNA content absence, Functional Assay (cDNA synthesis) |
Downloads
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