DBscript cDNA Synthesis Kit (DB-1285)
DBscript cDNA Synthesis Kit is an optimized kit containing all components needed for efficient synthesis of first-strand complementary DNA (cDNA) from mRNA or total RNA templates. Use our extremely fast protocol for your prompt analysis!
Complete cDNA synthesis with our unique bovine RNase inhibitor
DBscript Reverse Transcriptase: MMLV mutant with reduced RNase H activity for improved cDNA synthesis and increased yield and synthesis of longer cDNAs (> 9 kb).
Reverse transcription at temperatures from 50 °C to
60 °C due to a combination of increased thermostability of the protein and a unique buffer formulation.
Optimized short protocol (10 min) for two-step RT-PCR
Optimal protocol and buffer for one-step RT-PCR
DB RNase Inhibitor Bovine with exceptional oxidation resistance
Supplied with oligo(dT)20 primer, random hexamers, a reaction buffer, RT Buffer A (5X), and a separate DTT solution (20X).
Name (Cat. no.) | Quantity | Price | Lead Time |
DBScript cDNA Synthesis Kit (DB-1285) | 50 rxns | 180 EUR | In Stock |
Source, Unit Definition, QC and Storage Buffer
Source
Recombinant E. coli
Content
DBscript Reverse
Trancriptase (DB-1276)
DB RNase Inhibitor Bovine (DB-1260)
Oligo(dT)20 primer, random hexamers, a reaction buffer, RT Buffer A (5X), and a separate DTT solution (20X)
Storage Buffer
20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40, 50 % glycerol
QC Assays
SDS-PAGE/purity, DNase, RNase, latent RNase, endonuclease, DNA content absence
Functional Assay (cDNA synthesis)
Downloads
Order DBScript cDNA Synthesis Kit or ask for a free sample:
Markéta Stará
Sales Manager
