DBscript Reverse Transcriptase (DB-1276)
DBscript Reverse Transcriptase is essential for the synthesis of the complementary DNA (cDNA) strand from the RNA template. Use our Reverse Transcriptase in both two-step and one-step RT-PCR to ensure excellent results in your applications.
Superfast protocol with reduced pipetting steps
MMLV mutant with reduced RNase H activity for improved cDNA synthesis and increased yield and synthesis of longer cDNAs (> 9 kb).
Reverse transcription at temperatures from 50 °C to 60 °C due to a combination of increased thermostability of the protein and a unique buffer formulation.
Optimized short protocol (10 min) for two-step RT-PCR
Optimal protocol and buffer for one-step RT-PCR
Recombinant protein expressed in E. coli
Supplied with RT buffer (5X) including dNTPs and a separate DTT solution.
Package size: 2 kU; 10 kU; 40 kU
Name (Cat. no.) | Quantity | Price | Lead Time |
DBscript Reverse Transcriptase (DB-1276) | 2 kU | 40 EUR | In Stock |
DBscript Reverse Transcriptase (DB-1276) | 10 kU | 135 EUR | In Stock |
DBscript Reverse Transcriptase (DB-1276) | 40 kU | 385 EUR | In Stock |
Source, Unit Definition, QC and Storage Buffer
Source
Recombinant E. coli
Unit Definition
One unit is defined as the amount of enzyme that catalyzes incorporation of 1 nmol dNTPs (detected with SYBR® Green I dye) into
100 nM extendable DNA-RNA heteroduplex hairpin within 10 min at 37 °C.
Storage Buffer
20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40, 50 % glycerol
QC Assays
SDS-PAGE/purity
DNase, RNase, Endonuclease, DNA content
Functional Assay (cDNA synthesis)
Downloads
Order DBScript Reverse Transcriptase or ask for a free sample:
Markéta Stará
Sales Manager
