(DB-1276)
DBscript Reverse Transcriptase ensures excellent cDNA synthesis from RNA templates in both one-step and two-step RT-PCR applications. With a superfast protocol and reduced pipetting steps, it features an MMLV mutant with reduced RNase H activity for improved yield and longer cDNAs (> 9 kb). It supports reverse transcription at 50-60 °C, thanks to increased thermostability and a unique buffer formulation. Expressed as a recombinant protein in E. coli, it comes with a 5X RT buffer, including dNTPs and a separate DTT solution.
Package size
Key Parameters: Superior cDNA Synthesis
Superfast Protocol: Reduced pipetting steps for streamlined workflows
Enhanced cDNA Synthesis: MMLV mutant with reduced RNase H activity for improved yield and longer cDNAs (> 9 kb)
High-Temperature Stability: Reverse transcription at 50-60 °C due to increased thermostability and unique buffer formulation
Complete Kit: Supplied with 5X RT buffer, including dNTPs and a separate DTT solution
Pricing
Name (Cat. no.) | Quantity | Price | Lead Time |
|---|---|---|---|
DBscript Reverse Transcriptase (DB-1276) | 2 kU | 40 EUR | In Stock |
DBscript Reverse Transcriptase (DB-1276) | 10 kU | 135 EUR | In Stock |
DBscript Reverse Transcriptase (DB-1276) | 40 kU | 385 EUR | In Stock |
Details
Source | Unit Definition | Storage Buffer | QC Assays |
|---|---|---|---|
Recombinant E. coli | One unit is defined as the amount of enzyme that catalyzes incorporation of 1 nmol dNTPs (detected with SYBR® Green I dye) into 100 nM extendable DNA-RNA heteroduplex hairpin within 10 min at 37 °C. | 20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40, 50 % glycerol | SDS-PAGE/purity DNase, RNase, Endonuclease, DNA content Functional Assay (cDNA synthesis) |
Downloads
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