Oligonucleotide Synthesis

We have unique expertise and capabilities in customized DNA, RNA (including siRNA) or LNA oligonucleotide synthesis, offering:


Standard probe modifications

  • TaqMan Probes, different dyes, internal or terminal quenchers, oligo branching and click chemistry

Special modifications:

  • Azides, alkynes trans cyclooctenes, unorthodox dyes, modified bases -2′-O-Methyl, 2′-Fluoro, LNA

All of those with large scale production capability. See the complete list of modifications in this link!

Custom Oligonucleotide Synthesis

At DIANA Biotechnologies, we deliver top-quality custom oligonucleotides tailored to your needs—whether DNA, RNA (siRNA), or LNA. With a wide range of modifications, from TaqMan Probes to specialized bases and dyes, we guarantee the highest purity, quality, and yield at highly competitive prices.


Not sure which modification fits your project? Our team of experts is here to help! With personalized support, we’ll guide you through selecting the perfect dyes, quenchers, or modifications. Just fill out our form, and we'll get back to you right away with the answers you need!

We will provide you with complete customer support

Do you have any doubts of how to use your oligonucleotides? Are you unsure which dye or quencher you need to use? Our team of experts is here to help you. We offer a highly professional and flexible support! Fill out our form with everything you need to know about oligonucleotides and their applications and we will reach you immediately!

Price Calculator

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Chimeric type upon request

General information

Sequence (with or without modifications)

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Length 5-60

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Method of transport

In aqueous solution

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Technology

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siRNA


siRNAs are used in molecular biology to silence gene expression post-transcriptionally and are widely used in functional genomics studies, therapeutic development, and drug target validation. Their ability to selectively silence genes makes siRNAs a powerful tool for understanding gene function and developing treatments for various diseases, including cancers, viral infections, and genetic disorders.

LNA


LNA’s unique chemical structure enhances the thermal stability and binding affinity to their complementary DNA or RNA sequences resulting in increased specificity and resistance to nuclease degradation compared to traditional DNA or RNA molecules. These properties make LNAs highly effective in applications such as antisense therapy, gene silencing, microRNA research, and in situ hybridization. Additionally, LNA probes are used in quantitative PCR (qPCR) assays to improve detection sensitivity and accuracy. Increased maximum annealing temperature and improved specifity and affinity in base-pairing

TaqMan Probes


TaqMan™ probes is a key technology to your PCR assays providing high specificity, sensitivity, and quantification accuracy making them indispensable in research and diagnostics. We offer a wide range of dyes and quenchers at a highy competitive price.

  • All standard TaqMan dyes (FAM, HEX, ROX, TEX, Cy5, Cy5.5)
  • BHQ1 and BHQ2 terminal and internal or on the backbone or linked to the base

Other modifications


Thiol Chemistry Modifiers: Maleinimid, Vinyl sulfons, thiol modified 5′ end

Click Chemistry: Copper catalyzed clicked reaction, copper-free chemistry, Alkyne, Azide, PEGn-alkyne, PEGn-Azide

Spacers: Both internal and terminal with any desired lenght

NHS Reactive Modifiers: Aminomodifiers, both ends or internal

Non-standard bases: Terminator bases (either fluoro or deoxy)

All modifications listed can be performed on the 3′ end, 5′ end or internally.

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Let’s take your PCR to the next level!