(DB-1328)
Ensure excellent results in your PCR and RT-PCR applications with DB SuperSens Taq DNA Polymerase.
Our proprietary DB SuperSens Taq DNA Polymerase is designed for both routine and high-demanding applications. With high thermostability, sensitivity and specificity they are perfect for high-throughput PCR, TA cloning, and DNA labeling, this thermostable enzyme (half-life over 40 minutes at 95 °C) features an antibody-based hot-start for easy preparation at ambient temperature. It provides 5’→3′ polymerase and exonuclease activities, essential for hydrolysis probes like TaqMan™, and generates PCR products with 3′-dA overhangs for TA cloning.
Package size
Key Parameters: Achieve Superior PCR and RT-PCR Results with DB SuperSens Taq DNA Polymerase
Thermostable Taq DNA Polymerase: Recombinant enzyme with half-life of over 40 min at 95℃. Suitable for amplification up to 5 kb.Thermus aquaticus with a half-life of over 40 minutes at 95 °C.
Antibody-Based Hot-Start: Highly sensitive and specific hot-start ensure superior PCR specificity and sensitivity.long-term sample storage.
Three enzymatic Activities: Includes 5’→3′ polymerase activity, 5’→3′ exonuclease activity (essential for use with hydrolysis probes like TaqMan™), and 5′ flap activity.E. coli: no risk of transmission of infectious agents.
Wide range of applications: qPCR from cDNA, genomic DNA, plasmid DNA or synthesis of DNA templates for T/A cloning.
Pricing
Name (Cat. no.) | Quantity | Price | Lead Time |
|---|---|---|---|
DB SuperSens Taq DNA Polymerase (DB-1328) | 0.4 kU | 95 EUR | In Stock |
DB SuperSens Taq DNA Polymerase (DB-1328) | 1.0 kU | 207 EUR | In Stock |
DB SuperSens Taq DNA Polymerase (DB-1328) | 5.0 kU | 806 EUR | In Stock |
Details
Source | Unit Definition | Storage Buffer | QC Assays |
|---|---|---|---|
Recombinant E. coli | One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol of dNTPs into 250 nM fluorophore-quencher dual-labelled extendable DNA molecular beacon within 30 min at 72 °C. | 20 mM Tris pH 8.0, 100 mM KCl, 0.1 mM EDTA pH 8.0, 1 mM DTT, 0.5 % Igepal, 0.5 % Tween 20, 1 mg/mL BSA, 50 % v/v glycerol | SDS-PAGE/purity Functional Assay (PCR) Hot-start test (PCR) |
Downloads
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