RT-PCR SYBR Mixes

RT-PCR SYBR Mixes

DB RT-PCR SYBR mixes contain SYBR™ Green I dye for real-time quantification of RNA sequences and offer an ideal solution for routine and high-throughput RNA target detection. Afferdable RT-PCR – add only primers and template into the final reaction and get ready for our unique PCR technologies: DBdirect™ and SuperSens!

This system has been used as a basis in millions of diagnostic tests against COVID-19 including direct detection from samples of human saliva or standard collection media without the need for a previous RNA isolation step. Example of direct detection of viral RNA in cell culture is described in 10.1021/acsinfecdis.0c00829.

RT-PCR SYBR Mixes

DB RT-PCR SYBR Mix (DB-1264)

Ready to use RT- PCR SYBR Mix: 

  • Real-Time RT-PCR
  • SYBR™ Green I dye included in the mix: No TaqMan probes are needed
  • Hot-start: Aptamer
  • Various applications including quantitative real-time RT-PCR from RNA, cDNA, DNA
  • Detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media)

DBdirect™ RT-PCR SYBRMix (DB-1265)

RT-PCR with no need for NA extraction: Faster and more streamlined workflows

  • Real-Time RT-PCR
  • For those seeking unparalleled sensitivity in purified DNA detection
  • SYBR™ Green I dye included in the mix: No TaqMan probes are needed
  • Hot-start: Aptamer
  • Various applications including quantitative real-time RT-PCR from RNA, cDNA, DNA
  • Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media)

DB RT-PCR SYBR Mix SuperSens (DB-1268)

Direct PCR: No need for NA isolation with faster and more streamlined workflows

  • Real-Time RT-PCR
  • DBdirect™ technology: No need for NA isolation saves your time
  • SYBR™ Green I dye included in the mix: No TaqMan probes are needed
  • Hot-start: Aptamer
  • Various applications including quantitative real-time RT-PCR from RNA, cDNA, DNA
  • Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media)

DBdirect™ RT-PCR SYBR Mix SuperSens (DB-1269)

Direct PCR SuperSens: Advantage of direct PCR with exceptional sensitivity

  • Real-Time RT-PCR
  • For those seeking unparalleled sensitivity in purified DNA detection
  • SYBR™ Green I dye included in the mix: No TaqMan probes are needed
  • Hot-start: Antibody
  • Various applications including quantitative real-time RT-PCR from RNA, cDNA, DNA
  • Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media)
  • Suitable for single-cell detection

Key Protein, Hot-start, Content and QC

Key protein

Taq DNA Polymerase
Thermostable reverse transcriptase (mutated M-MLV)
RNase Inhibitors

Hot-start

Aptamer-based
(DB-1262, DB-1263)
Antibody-based
(DB-1266, DB-1267)

Content

Enhancer mix (4x)
RT-PCR Probe Mix (4x)/ DBdirect
™ PCR Probe Mix (4x)
RT-PCR Probe Mix SuperSens (4x)/ DBdirect™ PCR Probe Mix SuperSens (4x)

PCR Grade Water

QC Assays

Functional test (RT-PCR)
E. coli gDNA
RNase and DNase activity

Order DBdirect™ PCR SYBR Mixes or ask for a free sample:

    DB-1270-100RXNS
    DB-1270-1000RXNS
    DB-1273-100RXNS
    DB-1273-1000RXNS

    Markéta Stará

    Export Manager 

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