RT-PCR Probe Mixes

DB RT-PCR Probe mixes are designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. Save your time with our PCR Probe mixes by analyzing multiple targets simultaneously in one reaction. Get ready for our unique PCR technologies: DBdirect™ and SuperSens!

This system has been used as a basis in millions of diagnostic tests against COVID-19 including direct detection from samples of human saliva or standard collection media without the need for a previous RNA isolation step. Example of direct detection of viral RNA in cell culture is described in 10.1021/acsinfecdis.0c00829.

RT-PCR Probe Mixes_Price Table

DB RT-PCR Probe Mix (DB-1262)

Ready to use RT- PCR Probe Mix: 

  • Real-Time RT-PCR
  • Analyze multiple targets in one reaction
  • Hot-start: Aptamer
  • Various applications including quantitative real-time RT-PCR from RNA, cDNA, DNA
  • Detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media)
  • Not suitable for single-cell detection

DBdirect™ RT-PCR Probe Mix (DB-1263)

RT-PCR with no need for NA extraction: Faster and more streamlined workflows

  • Real-Time RT-PCR
  • DBdirect™: Direct detection with no NA isolation step
  • Analyze multiple targets in one reaction
  • Hot-start: Aptamer
  • Various applications including quantitative real-time RT-PCR from RNA, cDNA, DNA
  • Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media)
  • Suitable for single-cell detection

DB RT-PCR Probe Mix SuperSens (DB-1266)

  • Ready to use RT- PCR Probe Mix with SuperSens technology: 

    • Real-Time RT-PCR
    • Analyze multiple targets in one reaction
    • Hot-start: Antibody
    • Various applications including quantitative
      real-time RT-PCR from RNA, cDNA, DNA
    • Detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media)
    • Not suitable for single-cell detection
    •  

DBdirect™ RT-PCR Probe Mix SuperSens (DB-1267)

DBdirect with SuperSens: Ultimate solution for fast and exceptional sensitive analysis

  • Real-Time RT-PCR
  • DBdirect™: Direct detection with no NA isolation step
  • Analyze multiple targets in one reaction
  • Hot-start: Antibody
  • Various applications including quantitative real-time RT-PCR from RNA, cDNA, DNA
  • Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media)
  • Suitable for single-cell detection

Key Protein, Hot-start, Content and QC

Key protein

Taq DNA Polymerase
Thermostable reverse transcriptase (mutated M-MLV)
RNase Inhibitors

Hot-start

Aptamer-based
(DB-1262, DB-1263)
Antibody-based
(DB-1266, DB-1267)

Content

Enhancer mix (4x)
RT-PCR Probe Mix (4x)/ DBdirect
™ PCR Probe Mix (4x)
RT-PCR Probe Mix SuperSens (4x)/ DBdirect™ PCR Probe Mix SuperSens (4x)

PCR Grade Water

QC Assays

Functional test (RT-PCR)
E. coli gDNA
RNase and DNase activity

Order DBdirect™ PCR SYBR Mixes or ask for a free sample:

    DB-1270-100RXNS
    DB-1270-1000RXNS
    DB-1273-100RXNS
    DB-1273-1000RXNS

    Markéta Stará

    Export Manager 

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