DIANA is a multi-well plate detection assay, where target protein is selectively captured to the plate and then incubated with a unique detection probe consisting of a small-molecule inhibitor linked to a DNA-oligo. The amount of bound probe is quantified by quantitative PCR. It can be used either in a simple detection mode applicable in clinical diagnostics or in a modified screening mode with the application in drug discovery.
DIANA assay - introductory video
DIANA for diagnostics - protocol
4-step assay protocol which is easy to implement in a standard laboratory setting and compatible with CE IVD certification
DIANA in drug discovery - protocol
4-step protocol which is easy to implement in a standard laboratory setting, but it can be also automated for High-Throughput-Screening