DB AptaTaq DNA Polymerase (DB-1277)

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DB AptaTaq DNA Polymerase (DB-1277)

Use our DB AptaTaq DNA Polymerase in both PCR and RT-PCR to ensure excellent results in your applications (routine PCR amplification, high throughput PCR, TA cloning, DNA labelling)

DB AptaTaq DNA Polymerase

Thermostable Taq DNA Polymerase with aptamer-based hot-start

Highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus (half-life > 40 min at 95 °C)

Activities: Polymerase activity in direction 5’→3′ (synthesis of DNA), exonuclease activity in direction 5’→3′ (required for use in PCR with hydrolysis probes, e.g. TaqMan™ probes) and 5′ flap activity. No detectable exonuclease activity in the direction 3’→5′ (proofreading).

Aptamer-based hot-start for user-friendly preparation of PCR mix at ambient temperature.

Generation of PCR products with 3′ -dA overhangs for their use in TA cloning.

Recombinant protein expressed in E. coli

Supplied with complete PCR buffer (5X) including dNTPs and optimal concentration of MgCl2.

Name (Cat. no.)
Quantity
Price
Lead Time
DB AptaTaq DNA Polymerase (DB-1277)
0.1 kU
20 EUR
In Stock
DB AptaTaq DNA Polymerase (DB-1277)
0.5 kU
80 EUR
In Stock
DB AptaTaq DNA Polymerase (DB-1277)
2.5 kU
330 EUR
In Stock

Source, Unit Definition, QC and Storage Buffer

Source

Recombinant E. coli

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol of dNTPs into 250 nM fluorophore-quencher dual-labelled extendable DNA molecular beacon within 30 min at 72 °C.

Storage Buffer

20 mM Tris 8, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Igepal
CA-630, 0.5% Tween, 50% glycerol

QC Assays

SDS-PAGE/purity
Functional Assay (PCR)
DNase, RNase, Endonuclease activity absence
DNA content absence
E. coli gDNA absence

Downloads

Order DB AptaTaq DNA Polymerase or ask for a free sample:

    0.1 kU
    0.5 kU
    2.5 kU

    Aleš Pauk

    Head of Sales 

    Aleš Pauk Sales Manager